ProSci

RFI Antibody

Product Code:
 
PSI-46-283
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Caprine (Goat)
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
Storage:
 
Aliquot and store at -20˚C. Minimize freezing and thawing.
1 / 1
46-283 (4ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.
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46-283 (4ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.

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PSI-46-283-0.1mg0.1mg£626.00
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This product comes from: United States.
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Further Information

Additional Names:
RNF34, ring finger protein 34, RFI, RIF, RIFF, FLJ21786, RING finger protein RIFF, FYVE-RING finger protein MOMO, hRFI, CARP1, FLJ21786, caspases-8 and -10-associated RING finger protein 1
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:No signal obtained yet but low background observed in Human Testis lysate at up to 1ug/ml. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates?Immunohistochemistry:In paraffin embedded Human Kidney shows cytoplasm staining in select DCT. Recommended concentration, 4-8ug/ml.
Background References:
  • Sasaki S, Nakamura T, Arakawa H, Mori M, Watanabe T, Nagawa H, Croce CM. Isolation and characterization of a novel gene, hRFI, preferentially expressed in esophageal cancer. Oncogene. 2002 Jul 25;21(32):5024-30.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: KAGATSMWASCC
NCBI Gene ID #:
80196
NCBI Official Name:
ring finger protein 34, E3 ubiquitin protein ligase
NCBI Official Symbol:
RNF34
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_919247.1, NP_079402.2
Protein GI Number:
37595539
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
This antibody is expected to recognize both reported isoforms (NP_919247.1 and NP_079402.2).
Swissprot #:
Q969K3