ProSci

SEPT2 Antibody

Product Code:
 
PSI-46-756
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Caprine (Goat)
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
 
Aliquot and store at -20˚C. Minimize freezing and thawing.
1 / 4
46-756 (0.05ug/ml) staining of Human Testis lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
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4 / 4
46-756 (2.5ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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46-756 (0.05ug/ml) staining of Human Testis lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
46-756 (2.5ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

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PSI-46-756-0.1mg0.1mg£626.00
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Further Information

Additional Names:
SEPT2, septin 2, DIFF6, KIAA0158, NEDD5, Pnutl3, hNedd5, neural precursor cell expressed, developmentally down-regulated 5
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx. 40kDa band observed in Human Testis lysates (calculated MW of 41.5kDa according to NP_001008491.1). Recommended concentration: 0.05-0.2ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Breast, Tonsil and Brain (Cortex). Recommended concentration: 2.5ug/ml.
Background References:
  • Kremer BE, Adang LA, Macara IG. Septins regulate actin organization and cell-cycle arrest through nuclear accumulation of NCK mediated by SOCS7. Cell. 2007 Sep 7;130(5):837-50.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Cow
Immunogen:
The immunogen for this antibody is: SKQQPTQFINPET-C
NCBI Gene ID #:
4735
NCBI Official Name:
septin 2
NCBI Official Symbol:
Sep-02
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_001008491.1
Protein GI Number:
56549636
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer ,Signal Transduction
SPECIFICITY:
Reported variants represent identical protein: NP_001008492.1, NP_006146.1, NP_004395.1, NP_001008491.1.
Swissprot #:
Q15019