Further Information
POLL, BETA-N, POL-KAPPA, polymerase (DNA directed), lambda, polymerase (DNA-directed), lambda, DNA polymerase kappa (DNA polymerase beta-N), RP11-529I10.1, BETAN, FLJ46002, POLKAPPA, DNA polymerase beta-2, DNA polymerase kappa (DNA polymerase beta-N), DNA polymerase lamda2, DNA-directed DNA polymerase lambda, OTTHUMP00000020323, pol beta2, pol lambda
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 60kDa band observed in cell lysates of A549 and approx 60-70kDa in cell lysates of HepG2 (calculated MW of 63.5kDa according to Human NP_037406.1). Recommended concentration: 0.5-1ug/ml.
- Garcia-Diaz M, Bebenek K, Sabariegos R, Dominguez O, Rodriguez J, Kirchhoff T, Garcia-Palomero E, Picher AJ, Juarez R, Ruiz JF, Kunkel TA, Blanco L. DNA polymerase lambda, a novel DNA repair enzyme in human cells. J Biol Chem. 2002 Apr 12;277(15):13184-91.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
500 ug/mL
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
The immunogen for this antibody is: C-LGLPYREPAERDW
27343
polymerase (DNA directed), lambda
POLL
Homo sapiens
Liquid
NP_037406.1
7019491
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Cancer
It has been brought to our attention that this product may crossreact with Pol beta (NP_002681), an evolutionarilly related polymerase with a predicted size of 39 kDa. The overall % of identity is not very high (about 32% in the core) but considering only the peptide that we used as immunogen the two share the sequence YREP**R** at the C-terminus. Further the protein structures appear to be very similar in this region.
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