ProSci

PRAM1 Antibody

Product Code:
 
PSI-46-214
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Caprine (Goat)
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
 
Aliquot and store at -20˚C. Minimize freezing and thawing.
1 / 1
46-214 staining (2ug/ml) of Jurkat lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 12 hour. Detected by western blot using chemiluminescence.
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46-214 staining (2ug/ml) of Jurkat lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 12 hour. Detected by western blot using chemiluminescence.

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PSI-46-214-0.1mg0.1mg£626.00
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This product comes from: United States.
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Further Information

Additional Names:
PRAM-1, PRAM-1 protein, PML-RARA target gene encoding an Adaptor Molecule-1, PML-RARA regulated adaptor molecule 1, MGC39864, PML-RAR
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 90-100kDa band seen in Jurkat cell lysates. Recommended for use at 1-3ug/ml. Overnight incubation is recommended with this antibody. Please note that although the predicted MW of PRAM-1 is 79kDa, Moog-Lutz et al., (J. Biol. Chem, Jun 2001; 276: 22375 - 22381) detected aPRAM-1 migrating with an apparent molecular mass of 97 kDa and this is what we observed as well.
Background References:
  • Moog-Lutz C, Peterson EJ, Lutz PG, Eliason S, Cave-Riant F, Singer A, Di Gioia Y, Dmowski S, Kamens J, Cayre YE, Koretzky G. PRAM-1 is a novel adaptor protein regulated by retinoic acid (RA) and promyelocytic leukemia (PML)-RA receptor alpha in acute promyelocytic leukemia cells. J Biol Chem 2001 Jun 22;276(25):22375-81
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Pig
Immunogen:
The immunogen for this antibody is: DFCDPLENQPLPLGR
NCBI Gene ID #:
84106
NCBI Official Name:
PML-RARA regulated adaptor molecule 1
NCBI Official Symbol:
PRAM1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_115528.4
Protein GI Number:
302318952
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
.
Swissprot #:
Q96QH2