Further Information
ANLN, Scraps, ANILLIN, anillin, actin binding protein (scraps homolog, Drosophila), anillin (Drosophila Scraps homolog), actin binding protein, anillin, actin binding protein, DKFZp779A055, scra
Peptide ELISA: antibody detection limit dilution 1:64000.
Immunohistochemistry:In paraffin embedded Human Kidney shows staining of nuclei in some cells of renal tubules. Recommended concentration: 3-10ug/ml.
Immunofluorescence: Strong expression of the protein seen in the nuclei of U2OS cells. Recommended concentration: 10ug/ml.
Flow Cytometry: Flow cytometric analysis of MCF7 cells. Recommended concentration: 10ug/ml.
Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.
- Oegema K, Savoian MS, Mitchison TJ, Field CM. Functional analysis of a human homologue of the Drosophila actin binding protein anillin suggests a role in cytokinesis. J Cell Biol. 2000 Aug 7;150(3):539-52.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
500 ug/mL
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Expected Species Reactivity based on sequence homology: Mouse, Cow
The immunogen for this antibody is: WQPDACYKPIGKP
54443
anillin, actin binding protein
ANLN
Homo sapiens
Liquid
NP_061155.2, NP_001271230.1, NP_001271231.1
31657094
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Cancer
.
Q9NQW6