Further Information
ANK1 , ankyrin 1, erythrocytic , ANK, SPH1, SPH2, ankyrin 1, ankyrin-1, erythrocytic, ankyrin-R
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Preliminary experiments gave approx. 19-23 kDa bands in Human Skeletal Muscle lysates after 0.01ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for these bands we observe given the observed 28+30 kDa bands in the literature for this tissue (PMID: 9430667) and given the calculated size of 17.6 kDa according to NP_065211.2. The 19-22 kDa bands were successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
- Dubreuil RR. Functional links between membrane transport and the spectrin cytoskeleton. J Membr Biol. 2006;211(3):151-61. Epub 2006 Nov 7. Review.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
500 ug/mL
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Expected Species Reactivity based on sequence homology: Human, Mouse, Rat, Cow
The immunogen for this antibody is: C-QIVKRASLKRGKQ
286
ankyrin 1, erythrocytic
ANK1
Homo sapiens
Liquid
NP_000028.3, NP_065208.2, NP_065209.2, NP_065210.2, NP_065211.2, NP_065213.2
70780357
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Homeostasis
This antibody is expected to recognise isoform 3 (NP_000028.3) and isoform 5 (NP_065211.2).
P16157