ProSci

ANK1 Antibody

Product Code:
 
PSI-46-725
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Caprine (Goat)
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Enzyme-Linked Immunosorbent Assay (ELISA)
Storage:
 
Aliquot and store at -20˚C. Minimize freezing and thawing.
 

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PSI-46-725-0.1mg0.1mg£505.00
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Further Information

Additional Names:
ANK1 , ankyrin 1, erythrocytic , ANK, SPH1, SPH2, ankyrin 1, ankyrin-1, erythrocytic, ankyrin-R
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Preliminary experiments gave approx. 19-23 kDa bands in Human Skeletal Muscle lysates after 0.01ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for these bands we observe given the observed 28+30 kDa bands in the literature for this tissue (PMID: 9430667) and given the calculated size of 17.6 kDa according to NP_065211.2. The 19-22 kDa bands were successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Background References:
  • Dubreuil RR. Functional links between membrane transport and the spectrin cytoskeleton. J Membr Biol. 2006;211(3):151-61. Epub 2006 Nov 7. Review.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Human, Mouse, Rat, Cow
Immunogen:
The immunogen for this antibody is: C-QIVKRASLKRGKQ
NCBI Gene ID #:
286
NCBI Official Name:
ankyrin 1, erythrocytic
NCBI Official Symbol:
ANK1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_000028.3, NP_065208.2, NP_065209.2, NP_065210.2, NP_065211.2, NP_065213.2
Protein GI Number:
70780357
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Homeostasis
SPECIFICITY:
This antibody is expected to recognise isoform 3 (NP_000028.3) and isoform 5 (NP_065211.2).
Swissprot #:
P16157