Antibody Clonality:
Polyclonal
Applications:- Immunofluorescence (IF)
- Western Blot (WB)
Storage:
Store antibody at -20˚C for up to one year.
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![Western blot analysis of lysed extracts from 293 cells untreated or treated with PMA using MARCKS (Phospho-Ser162). Western blot analysis of lysed extracts from 293 cells untreated or treated with PMA using MARCKS (Phospho-Ser162).](https://www.prosci-inc.com/static-images/79-486.jpg)
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![Immunofluorescence staining of methanol-fixed HeLa cells using MARCKS (Phospho-Ser162). Immunofluorescence staining of methanol-fixed HeLa cells using MARCKS (Phospho-Ser162).](https://www.prosci-inc.com/static-images/79-486b.jpg)
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This product comes from:
United States.
Typical lead time:
14-21 working days.
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- Further Information
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Further Information
MACS, 80K-L, PKCSL, PRKCSL, TSFP1, MACS, MARCS, Protein kinase C substrate
Western Blot: 1:500~1:1000, Immunofluorescence: 1:100~1:200
MARCKS is the most prominent cellular substrate for protein kinase C. This protein binds calmodulin, actin, and synapsin. MARCKS is a filamentous (F) actin cross-linking protein.
- Pariser H, et al. Proc Natl Acad Sci U S A 2005 Aug 30; 102 (35): 12407-12412Nagumo H, et al. Biochem Biophys Res Commun 2001 Jan 26; 280 (3): 605-609Yamamoto H, et al. Arch Biochem Biophys 1998 Nov 15; 359 (2): 151-159
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
1 mg/mL
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
MARCKS (Phospho-Ser162) antibody was raised against a peptide sequence around phosphorylation site of serine 162 (K-K-S (p) -F-K) derived from Human MARCKS.
Phospho-Specific
4082
myristoylated alanine-rich protein kinase C substrate
MARCKS
Homo sapiens
Liquid
PREDICTED MOLECULAR WEIGHT:
80 kDa
NP_002347.5
153070260
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Phospho-Specific,Neuroscience
This antibody detects endogenous level of MARCKS only when phosphorylated at serine 162.
P29966