ProSci

VASP Antibody

Product Code:
 
PSI-79-583
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Rat
Applications:
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
 
Store antibody at -20˚C for up to one year.
1 / 2
Western blot analysis of lysed extracts from 293 and C6 cells using VASP (Ab-157).
2 / 2
Immunofluorescence staining of methanol-fixed HeLa cells using VASP (Ab-157).
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Western blot analysis of lysed extracts from 293 and C6 cells using VASP (Ab-157).
Immunofluorescence staining of methanol-fixed HeLa cells using VASP (Ab-157).

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PSI-79-583-0.1mg0.1mg£481.00
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Further Information

Additional Names:
VASP
Application Note:
Western Blot: 1:500~1:1000, Immunofluorescence: 1:100~1:200
Background:
Vasodilator-stimulated phosphoprotein (VASP) is a member of the Ena-VASP protein family. Ena-VASP family members contain an EHV1 N-terminal domain that binds proteins containing E/DFPPPPXD/E motifs and targets Ena-VASP proteins to focal adhesions. In the mid-region of the protein, family members have a proline-rich domain that binds SH3 and WW domain-containing proteins. Their C-terminal EVH2 domain mediates tetramerization and binds both G and F actin. VASP is associated with filamentous actin formation and likely plays a widespread role in cell adhesion and motility. VASP may also be involved in the intracellular signaling pathways that regulate integrin-extracellular matrix interactions. VASP is regulated by the cyclic nucleotide-dependent kinases PKA and PKG.
Background References:
  • Zhao WM, et al. (2001) EMBO J 20 (9): 2315-2325. Millard TH, et al. (2005) EMBO J 24 (2): 240-250. K?hnel K, et al. (2004) Proc Natl Acad Sci U S A 101 (49): 17027-17032. Haffner C, et al. (1995) EMBO J 14 (1): 19-27. Ramesh N, et al. (1997) Proc Natl Acad Sci U S A 94 (26): 14671-14676.
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
VASP (Ab-157) antibody was raised against a peptide sequence around aa.155~159 (R-V-S-N-A) derived from Human VASP.
NCBI Gene ID #:
7408
NCBI Official Name:
vasodilator-stimulated phosphoprotein
NCBI Official Symbol:
VASP
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
50 kDa
Protein Accession #:
NP_003361.1
Protein GI Number:
4507869
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Research Area:
Other
SPECIFICITY:
This antibody detects endogenous level of total VASP protein.
Swissprot #:
P50552