ProSci

CD8 Monoclonal Antibody

Product Code:
 
PSI-32-134
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Mouse
Antibody Isotype:
 
IgG1
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
RAVB3
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
 
CD8 monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Flow cytometric analysis of peripheral blood T cells using monoclonal antibody to human CD8.
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Flow cytometric analysis of peripheral blood T cells using monoclonal antibody to human CD8.

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PSI-32-134-0.1mg0.1mg£416.00
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Further Information

Additional Names:
MALCD8, MAL, p32, Leu2
Application Note:
Western Blot:1:500 - 1,000
ELISA:Determining optimal working dilutions by titration test.
Background:
CD8 T cell surface antigen is heterodimer of an alpha and a beta chain linked by two disulfide bonds .It belongs type I membrane protein. Selectively expressing of CD8 on a subset of T cells leads to CD8 T cell development. Through identifying cytotoxic/suppressor T-cells that interact with MHC class I bearing targets, CD8 is thought to play a role in the process of T-cell mediated killing. Veillette et al (1988) found the CD8 is associated with the internal membrane tyrosine-protein kinase p56lck.
Background References:
  • Veillette, A. et al. 1988. Cell 55:301.
Buffer:
Purified antibody in PBS containing 0.03% sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Ni-NTA purified recombinant human CD8 expressed in E. Coli strain BL21 (DE3).
NCBI Gene ID #:
925
NCBI Official Name:
CD8a molecule
NCBI Official Symbol:
CD8A
NCBI Organism:
Homo sapiens
Protein Accession #:
P01732
Protein GI Number:
116035
Purification:
Antibody is purified by protein A affinity chromatography.
Research Area:
Immunology ,
Swissprot #:
P01732
User NOte:
Optimal dilutions for each application to be determined by the researcher.