ProSci

HAP1 Antibody

Product Code:
 
PSI-45-721
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Caprine (Goat)
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
 
Aliquot and store at -20˚C. Minimize freezing and thawing.
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45-721 (0.1ug/ml) staining of Human Brain (Hippocampus) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
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45-721 (0.1ug/ml) staining of Human Brain (Hippocampus) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

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PSI-45-721-0.1mg0.1mg£626.00
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This product comes from: United States.
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Further Information

Additional Names:
HAP1, huntingtin-associated protein 1 (neuroan 1), HAP2, HIP5, HLP, hHLP1, huntingtin-associated protein 1, huntingtin-associated protein 2, neuroan 1
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 70kDa band observed in Human Brain (Frontal Cortex and Hippocampus) lysates (calculated MW of 69.6kDa according to NP_817084.1). Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour. An additional band of unknown identity was also consistently observed at 25kDa. This band was successfully blocked by incubation with the immunising peptide.Immunohistochemistry:Paraffin embedded Human Brain (Cortex). Recommended concentration: 3.75ug/ml.
Background References:
  • Takeshita Y, Fujinaga R, Zhao C, Yanai A, Shinoda K. Huntingtin-associated protein 1 (HAP1) interacts with androgen receptor (AR) and suppresses SBMA-mutant-AR-induced apoptosis. Hum Mol Genet. 2006 Aug 1;15(15):2298-312. Epub 2006 Jun 16.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-RYDFRYSEDREQ
NCBI Gene ID #:
9001
NCBI Official Name:
huntingtin-associated protein 1
NCBI Official Symbol:
HAP1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_003940.2, NP_817084.1
Protein GI Number:
30061496
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
This antibody is expected to recognize all reported isoforms (NP_003940.1, NP_817084.1)
Swissprot #:
P54257