ProSci

Neuro-d4 Antibody

Product Code:
 
PSI-45-954
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Caprine (Goat)
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
 
Aliquot and store at -20˚C. Minimize freezing and thawing.
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45-954 Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing strong nuclear and weak cytoplasmic staining. The
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45-954 Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing strong nuclear and weak cytoplasmic staining. The

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PSI-45-954-0.1mg0.1mg£626.00
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Further Information

Additional Names:
DPF1, NEUD4, neuro-d4, D4, zinc and double PHD fingers family 1, Neuro-d4 (rat) homolog, D4, zinc and double PHD fingers, family 1, MGC150428, MGC150429, BAF45b, OTTHUMP00000211615, neuro-d4 homolog
Application Note:
Peptide ELISA: antibody detection limit dilution 1:2000.Western Blot:Preliminary experiments showed a band at approx 38-40kDa in Human Cerebellum and Frontal Cortex lysates after 0.3-1ug/ml antibody staining (calculated MW of 37.9kDa according to NP_001128628.1. Primary incubation 1 hour at room temperature.Immunofluorescence: Strong expression of the protein seen in the nuclei and weaker staining in the cytoplasm of MCF7 and U2OS cells. Recommended concentration: 10ug/ml.
Background References:
  • Chestkov AV, Baka ID, Kost MV, Georgiev GP, Buchman VL. The d4 gene family in the human genome. Genomics. 1996 Aug 15;36(1):174-7.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Cow
Immunogen:
The immunogen for this antibody is: C-HLKEKASAYITLT
NCBI Gene ID #:
8193
NCBI Official Name:
D4, zinc and double PHD fingers family 1
NCBI Official Symbol:
DPF1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_001128627.1, NP_004638.2, NP_001128628.1
Protein GI Number:
4758798
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
This antibody is expected to recognize all three reported isoforms (NP_001128627.1; NP_004638.2; NP_001128628.1).
Swissprot #:
Q92782