ProSci

CNTF Antibody

Product Code:
 
PSI-XP-5114
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Neutralisation
  • Western Blot (WB)
Storage:
 
CNTF antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-t
1 / 6
To detect Rat CNTF by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci?s Biotinylated Anti-Rat CNTF (XP-5114Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat CNTF.
2 / 6
To detect Rat CNTF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat CNTF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
3 / 6
To detect Rat CNTF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat CNTF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
4 / 6
To detect Rat CNTF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat CNTF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
5 / 6
To detect Rat CNTF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat CNTF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
6 / 6
This antibody stained colchicine injected rat brain (including the globus pallidus region) tissue. The primary antibody was incubated at 0.25 mg/ml overnight at 4°C. This was followed by a peroxidase conjugated secondary antibody and then a fluorescein Tyramide Signal Amplification (TSA) reagent. Optimal concentrations and conditions may vary.
❮❯

To detect Rat CNTF by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci?s Biotinylated Anti-Rat CNTF (XP-5114Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat CNTF.
To detect Rat CNTF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat CNTF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
To detect Rat CNTF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat CNTF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
To detect Rat CNTF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat CNTF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
To detect Rat CNTF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat CNTF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
This antibody stained colchicine injected rat brain (including the globus pallidus region) tissue. The primary antibody was incubated at 0.25 mg/ml overnight at 4°C. This was followed by a peroxidase conjugated secondary antibody and then a fluorescein Tyramide Signal Amplification (TSA) reagent. Optimal concentrations and conditions may vary.

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PSI-XP-5114-0.1mg0.1mg£524.00
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Further Information

Additional Names:
Ciliary neurotrophic factorCNTF
Application Note:
Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of rCNTF (0.15 ng/mL), a concentration of 0.02 - 0.05 μg/mL of this antibody is required.

ELISA:

To detect rCNTF by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant rCNTF.

Sandwich: To detect Rat CNTF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Rat CNTF (XP-5114Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat CNTF.

Western Blot:
To detect rCNTF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant rCNTF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Background:
Ciliary neurotrophic factor (CNTF) is expressed in glial cells within the central and peripheral nervous systems. CNTF stimulates gene expression, cell survival or differentiation in a variety of neuronal cell types such as sensory, sympathetic, ciliary and motor neurons. CNTF itself lacks a classical signal peptide sequence of a secreted protein, but is thought to convey its cytoprotective effects after release from adult glial cells by some mechanism induced by injury.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant rCNTF (Rat Ciliary Neurotrophic Factor).
NCBI Gene ID #:
25707
NCBI Official Name:
ciliary neurotrophic factor
NCBI Official Symbol:
Cntf
NCBI Organism:
Rattus norvegicustus norvegicus
Physical State:
Lyophilized
Protein Accession #:
P20294
Protein GI Number:
116587
Purification:
Anti-rCNTF specific antibody was purified by affinity chromatography employing immobilized rCNTF matrix.
Research Area:
Chemokines & Cytokines,Antibody Pairs, Functional Assays
Swissprot #:
P20294
User NOte:
Centrifuge vial prior to opening.