ProSci

RIP3 (Rippy-3) Monoclonal Antibody

Product Code:
 
PSI-XA-1023
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG1
Antibody Clonality:
 
Monoclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Western Blot (WB)
Storage:
 
RIP3 antibody can be stored at 4˚C, stable for one year.
 

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PSI-XA-1023-0.1mg0.1mg£686.00
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Further Information

Application Note:
RIP3 antibody can be used to recognize human RIP3 by western blot. Method: HEK 293T cells?(5x105) mock transfected?(lane 1) or transfected (lane 2) with an expression plasmid coding for human RIP3 were harvested, lysed and cell extracts (5x105) were resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and incubated with the Rippy-3 monoclonal antibody at a 1:1000 dilution for 2 hours. Proteins were visualized using a peroxidase-conjugated antibody to mouse IgG and a chemiluminescence detection system. This product is for research use only.
Background References:
  • Identification of RIP3, a RIP-like kinase that activates apoptosis and NFkappaB: P.W. Yu, et al.; Curr. Biol. 9, 539 (1999).
  • RIP3, a novel apoptosis-inducing kinase: X. Sun, et al.; J. Biol. Chem. 274, 16871 (1999).
  • Mouse receptor interacting protein 3 does not contain a caspase-recruiting or a death domain but induces apoptosis and activates NF-kappaB: N.J. Pazdernik, et al.; Mol. Cell. Biol. 19, 6500 (1999).
  • RIP4 (DIK/PKK), a novel member of the RIP kinase family, activates NF-kappa B and is processed during apoptosis: E. Meylan, et al.; EMBO Rep. 3, 1201 (2002).
Buffer:
PBS containing 0.02% sodium azide
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
RIP3 (monoclonal Rippy-3) antibody was raised against recombinant human RIP3.
NCBI Gene ID #:
11035
NCBI Official Name:
receptor-interacting serine-threonine kinase 3
NCBI Official Symbol:
RIPK3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Purification:
RIP3 (monoclonal Rippy-3) antibody was purified to >=95% in SDS-PAGE.
Research Area:
Apoptosis
Swissprot #:
Q9Y572
User NOte:
Optimal dilutions for each application to be determined by the researcher.