ProSci

SRCAP Antibody

Product Code:
 
PSI-42-210
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Caprine (Goat)
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Application:
 
Enzyme-Linked Immunosorbent Assay (ELISA)
Storage:
 
Aliquot and store at -20˚C. Minimize freezing and thawing.
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42-210 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. The nuclear stain is DAPI (blue)
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42-210 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. The nuclear stain is DAPI (blue)

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PSI-42-210-0.1mg0.1mg£626.00
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This product comes from: United States.
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Further Information

Additional Names:
SRCAP, Snf2-related CREBBP activator protein, DOMO1, EAF1, FLJ44499, KIAA0309, SWR1, OTTHUMP00000163226, Snf2-related CBP activator protein, Swi2/Snf2-related ATPase homolog, domino homolog 1
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Not yet tested - our routine western blotting protocol does not allow for the detection of proteins >250kDa (calculated size of approx. 344kDa according to NP_006653.2).Immunofluorescence: Strong expression of the protein seen in the Nucleus of A31 cells. Recommended concentration: 10ug/ml.
Background References:
  • Wong MM, Cox LK, Chrivia JC. The chromatin remodeling protein, SRCAP, is critical for deposition of the histone variant H2A.Z at promoters. The Journal of biological chemistry 2007 Sep 282 (36): 26132-9.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog
Immunogen:
The immunogen for this antibody is: C-DEAQNIKNFKSQR
NCBI Gene ID #:
10847
NCBI Official Name:
Snf2-related CREBBP activator protein
NCBI Official Symbol:
SRCAP
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_006653.2
Protein GI Number:
146219843
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Swissprot #:
Q6ZRS2