ProSci

Collagen VII Antibody

Product Code:
 
PSI-46-647
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Caprine (Goat)
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
 
Aliquot and store at -20˚C. Minimize freezing and thawing.
1 / 1
46-647 (0.5ug/ml) staining of Rat Testis lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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46-647 (0.5ug/ml) staining of Rat Testis lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

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PSI-46-647-0.1mg0.1mg£626.00
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This product comes from: United States.
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Further Information

Additional Names:
Collagen, type VII, collagen, type VII, alpha 1 (epidermolysis bullosa, dystrophic, dominant and recessive), COL7A1, HGNC:2214, EBD1, EBDCT, EBR1, LC collagen, alpha 1 type VII collagen, collagen VII, alpha-1 polypeptide, long chain collagen, collagen, type VII, alpha 1
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 250kDa band observed in Rat Testis lysates (calculated MW of 159kDa according to Rat NP_001100328.1). Recommended concentration: 0.1-1.5ug/ml.A customer found this antibody unsuccessful on cryosections of Human Skin.
Background References:
  • Ortiz-Urda S, Garcia J, Green CL, Chen L, Lin Q, Veitch DP, Sakai LY, Lee H, Marinkovich MP, Khavari PA. Type VII collagen is required for Ras-driven human epidermal tumorigenesis. Science. 2005 Mar 18;307(5716):1773-6
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Human, Mouse, Dog, Pig
Immunogen:
The immunogen for this antibody is: C-TVQYSDDPRTEF
NCBI Gene ID #:
1294
NCBI Official Name:
collagen, type VII, alpha 1
NCBI Official Symbol:
COL7A1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_000085.1
Protein GI Number:
4502961
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Homeostasis
SPECIFICITY:
This product does not work on cryostat sections of Normal Human Skin.
Swissprot #:
Q02388