ProSci

PDK1 Antibody

Product Code:
 
PSI-25-028
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
 
For short periods of storage (days) store at 4˚C. For longer periods of storage, store PDK1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
1 / 3
Antibody used in WB on Human MCF-7 at 0.2-1 ug/ml.
2 / 3
3 / 3
Antibody used in WB on Human Jurkat 1 ug/ml.
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Antibody used in WB on Human MCF-7 at 0.2-1 ug/ml.
Antibody used in WB on Human Jurkat 1 ug/ml.

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PSI-25-028-100ul100ul£662.00
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Further Information

Additional Names:
PDK1, PDHK1
Application Note:
PDK1 antibody can be used for detection of PDK1 by ELISA at 1:1562500. PDK1 antibody can be used for detection of PDK1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Background:
Pyruvate dehydrogenase (PDH) is a mitochondrial multienzyme complex that catalyzes the oxidative decarboxylation of pyruvate and is one of the major enzymes responsible for the regulation of homeostasis of carbohydrate fuels in mammals. The enzymatic activity is regulated by a phosphorylation/dephosphorylation cycle. Phosphorylation of PDH by a specific pyruvate dehydrogenase kinase (PDK) results in inactivation.Pyruvate dehydrogenase (PDH) is a mitochondrial multienzyme complex that catalyzes the oxidative decarboxylation of pyruvate and is one of the major enzymes responsible for the regulation of homeostasis of carbohydrate fuels in mammals. The enzymatic activity is regulated by a phosphorylation/dephosphorylation cycle. Phosphorylation of PDH by a specific pyruvate dehydrogenase kinase (PDK) results in inactivation. Please see the Entrez Gene record to access additional publications.
Background References:
  • Ohba, T., (2008) Biochem. Biophys. Res. Commun. 369 (2), 376-381.
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PDK1.
NCBI Gene ID #:
5163
NCBI Official Name:
pyruvate dehydrogenase kinase, isozyme 1
NCBI Official Symbol:
PDK1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
47 kDa
Protein Accession #:
NP_002601
Protein GI Number:
4505689
Purification:
Antibody is purified by peptide affinity chromatography method.
Research Area:
Cancer ,Signal Transduction ,Immunology
Swissprot #:
Q15118
User NOte:
Optimal dilutions for each application to be determined by the researcher.