ProSci

SPP1 Antibody

Product Code:
 
PSI-28-547
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
 
For short periods of storage (days) store at 4˚C. For longer periods of storage, store SPP1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
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Antibody used in IHC on Human kidney.
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Antibody used in WB on Jurkat 2.5 ug/ml (Lane A: Primary Antibody and Lane B: Primary Antibody + Blocking Peptide ).
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Antibody used in IHC on Human kidney.
Antibody used in WB on Jurkat 2.5 ug/ml (Lane A: Primary Antibody and Lane B: Primary Antibody + Blocking Peptide ).

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PSI-28-547-100ul100ul£609.00
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Further Information

Additional Names:
SPP1, OPN, BNSP, BSPI, ETA-1
Application Note:
SPP1 antibody can be used for detection of SPP1 by ELISA at 1:1562500. SPP1 antibody can be used for detection of SPP1 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Background:
The SPP1 gene encodes an acidic matrix protein, mainly expressed in mineralized tissues, kidney and atherosclerotic vessels. This protein also contributes to several steps in the process of prostate carcinogenesis and metastasis.
Background References:
  • Iwata, M., et al., (2004) Blood 103 (12), 4496-4502.
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SPP1.
NCBI Gene ID #:
6696
NCBI Official Name:
secreted phosphoprotein 1
NCBI Official Symbol:
SPP1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
33 kDa
Protein Accession #:
NP_000573
Protein GI Number:
4759166
Purification:
Antibody is purified by protein A chromatography method.
Research Area:
Cancer ,Signal Transduction ,Immunology ,Apoptosis ,Primary ///Growth Factor ,
Swissprot #:
P10451-2
User NOte:
Optimal dilutions for each application to be determined by the researcher.