ProSci

FSTL1 Antibody

Product Code:
 
PSI-45-640
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Caprine (Goat)
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
 
Aliquot and store at -20˚C. Minimize freezing and thawing.
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45-640 (1ug/ml) staining of Human Ovary lysate (A) + peptide (B) (35ug protein in RIPA buffer). Detected by chemiluminescence.
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45-640 (1ug/ml) staining of Human Ovary lysate (A) + peptide (B) (35ug protein in RIPA buffer). Detected by chemiluminescence.

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PSI-45-640-0.1mg0.1mg£626.00
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Further Information

Additional Names:
FSTL1, follistatin-like 1, FRP, FSL1, follistatin-related protein, FLJ50214, FLJ52277,
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 50kDa band obseerved in Human Ovary lysates. (calculated MW of 35.0kDa according to NP_009016.1). This band corresponds to earlier findings with different antibodies from other commercial sources and was successfully blocked by incubation with the immunizing peptide. Recommended concentration: 0.3-1ug/ml. Primary incubation 1 hour at room temperature.Immunofluorescence: Strong expression of the protein seen in the cytoplasm and vesicles of A431 cells. Recommended concentration: 10ug/ml.

Flow Cytometry: Flow cytometric analysis of A549 cells. Recommended concentration: 10ug/ml.

Background References:
  • Tanaka M, Ozaki S, Osakada F, Mori K, Okubo M, Nakao K. Cloning of follistatin-related protein as a novel autoantigen in systemic rheumatic diseases. Int Immunol 1998 Sep;10(9):1305-14
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-TAEKTKRVSTKEI
NCBI Gene ID #:
11167
NCBI Official Name:
follistatin-like 1
NCBI Official Symbol:
FSTL1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_009016.1
Protein GI Number:
5901956
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
.
Swissprot #:
Q12841