Further Information
FRAP, FRAP1, FRAP2, RAFT1, RAPT1, Mek5, Mkk5, Prkmk5, FRAP
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Kinase subunit of both mTORC1 and mTORC2, which regulate cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. Amino-acid-signaling to mTORC1 is mediated by Rag GTPases, which cause amino-acid-induced relocalization of mTOR within the endomembrane system. Growth factor-stimulated mTORC1 activation involves AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-421', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. mTORC2 is also activated by growth factors, but seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'.
- Albanell J, et al. (2007) Clin Transl Oncol. 9 (8): 484-93. Huang JJ, et al. (2007) Ai Zheng. 26 (12): 1397-403. Kenerson H, et al. (2007) Hum Pathol. 38 (9): 1361-71.
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
1 mg/mL
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
mTOR (Ab-2448) antibody was raised against a peptide sequence around aa. 2446~2450 (T-D-S-Y-S) derived from Human mTOR.
2475
mechanistic target of rapamycin (serine/threonine kinase)
MTOR
Homo sapiens
Liquid
PREDICTED MOLECULAR WEIGHT:
289 kDa
NP_004949.1
4826730
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Cell Cycle
This antibody detects endogenous level of total mTOR protein.
P42345