ProSci

IRF5 Antibody

Product Code:
 
PSI-45-075
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Caprine (Goat)
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • ChIP
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunoprecipitation (IP)
  • Western Blot (WB)
Storage:
 
Aliquot and store at -20˚C. Minimize freezing and thawing.
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45-075 staining (1ug/ml) Human Spleen (A) and Peripheral Blood Lymphocytes (B) lysates (RIPA buffer, 30ug total protein per lane). Detected by chemiluminescence
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45-075 staining (1ug/ml) Human Spleen (A) and Peripheral Blood Lymphocytes (B) lysates (RIPA buffer, 30ug total protein per lane). Detected by chemiluminescence

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PSI-45-075-0.1mg0.1mg£626.00
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This product comes from: United States.
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Further Information

Additional Names:
IRF5, interferon regulatory factor 5, SLEB10
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 60kDa band observed in Human Spleen and Peripheral Blood Lymphocytes lysates.The calculated molecular size is 57.8kDa according to NP_001092099.1. Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:This antibody showed nuclear staining in Human Cervix, as confirmed by customer (data not shown). This data is from a previous batch, not on sale.Chromatin immunoprecipitation: This antibody has been successfully used in human monocyte-derived dendritic cells.This data is from a previous batch, not on sale.
Background References:
  • Barnes BJ, Moore PA, Pitha PM. Virus-specific activation of a novel interferon regulatory factor, IRF-5, results in the induction of distinct interferon alpha genes. J Biol Chem. 2001 Jun 29;276(26):23382-90.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Cow
Immunogen:
The immunogen for this antibody is: C-QGPWPMHPAGMQ
NCBI Gene ID #:
3663
NCBI Official Name:
interferon regulatory factor 5
NCBI Official Symbol:
IRF5
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_002191.1, NP_116032.1, NP_001092097.1, NP_001092099.1
Protein GI Number:
4504727
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Immunology ,Apoptosis
SPECIFICITY:
This antibody is expected to recognize all four reported isoforms (NP_002191.1; NP_116032.1; NP_001092097.1; NP_001092099.1). Reported variants NP_001092100.1, NP_001092101.1, NP_001092098.1, NP_001092097.1 represent identical protein: Reported variants NP_116032.1, NP_001092100.1 represent identical protein:
Swissprot #:
Q13568