ProSci

OCT4 Antibody

Product Code:
 
PSI-45-991
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Caprine (Goat)
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
 
Aliquot and store at -20˚C. Minimize freezing and thawing.
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45-991 (2ug/ml) staining of Human Placenta lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
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45-991 (2ug/ml) staining of Human Placenta lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

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CodeSizePrice
PSI-45-991-0.1mg0.1mg£626.00
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This product comes from: United States.
Typical lead time: 14-21 working days.
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Further Information

Additional Names:
POU5F1, OTF4, POU domain, class 5, transcription factor 1, HGNC:9221, MGC22487, OCT3, OTF3, POU-type homeodomain-containing DNA-binding protein, octamer-binding transcription factor-3, POU class 5 homeobox 1, DADB-104B20.2, OCT4, POU domain, class 5, transcription factor 1
Application Note:
Peptide ELISA: antibody detection limit dilution 1:2000.Western Blot:Approx 30kDa band observed in Human Placenta, and approx 28kDa in Mouse Heart lysates (calculated MW of 30.1kDa according to Human NP_976034.3 and 25.0kDa according to Mouse NP_001239381.1). Recommended concentration: 0.5--2ug/ml. Primary incubation was 1 hour
Background References:
  • Jones TD, Ulbright TM, Eble JN, Baldridge LA, Cheng L. OCT4 staining in testicular tumors: a sensitive and specific marker for seminoma and embryonal carcinoma. Am J Surg Pathol. 2004 Jul;28(7):935-40.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Rat, Dog
Immunogen:
The immunogen for this antibody is: C-VTTLGSPMHSN
NCBI Gene ID #:
5460
NCBI Official Name:
POU class 5 homeobox 1
NCBI Official Symbol:
POU5F1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_002692.2, NP_976034.3
Protein GI Number:
42560248
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
This antibody is expected to recognise both reported isoforms (NP_002692.2 and NP_976034.3)
Swissprot #:
Q01860