ProSci

Ramp1 Antibody

Product Code:
 
PSI-46-890
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Caprine (Goat)
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
 
Aliquot and store at -20˚C. Minimize freezing and thawing.
1 / 1
46-890 (0.3ug/ml) staining of Rat Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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46-890 (0.3ug/ml) staining of Rat Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

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PSI-46-890-0.1mg0.1mg£626.00
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Further Information

Additional Names:
Ramp1, receptor (calcitonin) activity modifying protein 1, 9130218E19Rik, MGC38240, receptor-activity modifying protein 1
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Experiments gave bands at approx. 18kDa and 40kDa in Rat Brain lysate after 0.3ug/ml antibody staining. The appearance of a band with much higher molecular weight corresponds to earlier findings in literature with different antibodies (Hilairet, J Biol Chem. 2001 Nov 9;276(45):42182-90.; PMID: 11535606). This protein has a calculated MW of 16.9kDa according to Mouse NP_058590.1 and 16.8kDa according to Rat NP_113833.1. Recommended concentration: 0.3-1ug/ml.
Background References:
  • Tsujikawa K, Yayama K, Hayashi T, Matsushita H, Yamaguchi T, Shigeno T, Ogitani Y, Hirayama M, Kato T, Fukada S, Takatori S, Kawasaki H, Okamoto H, Ikawa M, Okabe M, Yamamoto H. Hypertension and dysregulated proinflammatory cytokine production in receptor activity-modifying protein 1-deficient mice. Proc Natl Acad Sci USA. 2007 Oct 16;104(42):16702-7
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Human, Mouse,
Immunogen:
The immunogen for this antibody is: C-DYQSKRTEGIV
NCBI Gene ID #:
10267
NCBI Official Name:
receptor (G protein-coupled) activity modifying protein 1
NCBI Official Symbol:
Ramp1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_058590.1, NP_848488.2
Protein GI Number:
8394145
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Signal Transduction ,Immunology
SPECIFICITY:
This antibody is expected to recognize isoform 1 (NP_058590.1) only.
Swissprot #:
O60894