ProSci

RXR alpha Antibody

Product Code:
 
PSI-45-139
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Caprine (Goat)
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
 
Aliquot and store at -20˚C. Minimize freezing and thawing.
1 / 1
45-139 (0.3ug/ml) staining of nuclear HeLa lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
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45-139 (0.3ug/ml) staining of nuclear HeLa lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

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PSI-45-139-0.1mg0.1mg£626.00
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Further Information

Additional Names:
RXRA, NR2B1, retinoid X receptor, alpha, FLJ16020, FLJ16733, MGC102720, FLJ00280, FLJ00318,
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 60kDa band observed in nuclear lysates of cell line HeLa (calculated MW of 50.8kDa according to NP_002948.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Solomon et al, J Biol Chem. 1998 Jul 10;273(28):17573-8; PMID: 9651351. Recommended concentration: 0.3-1ug/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on NIH3T3 cell line for this particular batch.
Background References:
  • Claudel T, Leibowitz MD, Fievet C, Tailleux A, Wagner B, Repa JJ, Torpier G, Lobaccaro JM, Paterniti JR, Mangelsdorf DJ, Heyman RA, Auwerx J. Reduction of atherosclerosis in apolipoprotein E knockout mice by activation of the retinoid X receptor. Proc Natl Acad Sci USA. 2001 Feb 27;98(5):2610-5. Epub 2001 Feb 20.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat
Immunogen:
The immunogen for this antibody is: CQVNSSLTSPTGRGSM
NCBI Gene ID #:
6256
NCBI Official Name:
retinoid X receptor, alpha
NCBI Official Symbol:
RXRA
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_002948.1
Protein GI Number:
4506755
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Homeostasis
SPECIFICITY:
This antibody is expected to recognise an epitope corresponding to aa 14-28 of human RXR alpha protein and does not cross-react with either RXR beta or gamma.
Swissprot #:
P19793