ProSci

Trem2 Antibody

Product Code:
 
PSI-42-134
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Caprine (Goat)
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
 
Aliquot and store at -20˚C. Minimize freezing and thawing.
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42-134 Immunofluorescence analysis of paraformaldehyde fixed 3T3-L1 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane/cytoplasmic staining. The nuclear stain
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42-134 Immunofluorescence analysis of paraformaldehyde fixed 3T3-L1 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane/cytoplasmic staining. The nuclear stain

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PSI-42-134-0.1mg0.1mg£626.00
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Further Information

Additional Names:
Trem2, triggering receptor expressed on myeloid cells 2 , Trem2a, Trem2b, Trem2c , triggering receptor expressed on myeloid cells 2a, triggering receptor expressed on myeloid cells 2b, triggering receptor expressed on myeloid cells 2c
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments showed a band at approx 26kDa in Mouse and Rat Adrenal gland lysates after 1ug/ml antibody staining (calculated MW of 24.5kDa according to Mouse NP_112544.1 and 24.9 according to Rat NP_001100354.1). Primary incubation 1 hour at room temperature.Immunofluorescence: Strong expression of the protein seen in the Plasma Membrane of NIH3T3-L1 (adipocyte;like) cells. Recommended concentration: 10ug/ml.
Background References:
  • Takahashi K, Prinz M, Stagi M, Chechneva O, Neumann H. TREM2-transduced myeloid precursors mediate nervous tissue debris clearance and facilitate recovery in an animal model of multiple sclerosis. PLoS Med. 2007 Apr;4(4):e124.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Rat
Immunogen:
The immunogen for this antibody is: C-QVEHSTSRNQET
NCBI Gene ID #:
83433 (mouse); 301227 (rat);
NCBI Official Name:
triggering receptor expressed on myeloid cells 2
NCBI Official Symbol:
Trem2
NCBI Organism:
Mus musculus
Physical State:
Liquid
Protein Accession #:
NP_112544.1
Protein GI Number:
13994125
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
.
Swissprot #:
Q99NH8