ProSci

CBR3 Antibody

Product Code:
 
PSI-45-369
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Caprine (Goat)
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Enzyme-Linked Immunosorbent Assay (ELISA)
Storage:
 
Aliquot and store at -20˚C. Minimize freezing and thawing.
1 / 1
45-369 (0.5ug/ml) staining of HEK293 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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45-369 (0.5ug/ml) staining of HEK293 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

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PSI-45-369-0.1mg0.1mg£626.00
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Further Information

Additional Names:
CBR3, carbonyl reductase 3, carbonyl reductase (NADPH) 3, SDR21C2, hCBR3, NADPH-dependent carbonyl reductase 3, short chain dehydrogenase/reductase family 21C, member 2
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 35kDa band observed in lysates of cell line HEK293 (calculated MW of 30.9kDa according to NP_001227.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Bains et al, J Pharmacol Exp Ther. 2010 Mar;332(3):755-63.; PMID: 20007405). Recommended concentration: 1-3ug/ml. An anonymous customer found positive results in WB on Human recombinant protein.
Background References:
  • Watanabe K, Sugawara C, Ono A, Fukuzumi Y, Itakura S, Yamazaki M, Tashiro H, Osoegawa K, Soeda E, Nomura T. Mapping of a novel human carbonyl reductase, CBR3, and ribosomal pseudogenes to human chromosome 21q22.2. Genomics. 1998 Aug 15;52(1):95-100.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: C-QGQLVHDKVVQNW
NCBI Gene ID #:
874
NCBI Official Name:
carbonyl reductase 3
NCBI Official Symbol:
CBR3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_001227.1
Protein GI Number:
4502601
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
Swissprot #:
O75828