Further Information
GABPB2, GA binding protein transcription factor, beta subunit 2, BABPB2, E4TF1, E4TF1-47, E4TF1-53, E4TF1B, GABPB, GABPB1, NRF2B1, NRF2B2, GA binding protein transcription factor, beta subunit 1, 53kDa, GA binding protein transcription factor, beta subunit 2, 47kDa, nuclear respiratory factor-2 subunit beta
Peptide ELISA: antibody detection limit dilution 1:32000.
Western Blot:Approx 45+38kDa bands observed in lysates of cell line HeLa (calculated MW of 42.5kDa according to NP_005245.2, NP_001307844.1 and of 38.1kDa according to NP_002032.2). An additional band of unknown identity was also consistently observed at 22kDa and was successfully blocked by incubation with the immunising peptide. Recommended concentration: 0.3-1ug/ml. Primary incubation 1 hour at room temperature.
Immunohistochemistry:Paraffin embedded Human Tonsil and Spleen. Recommended concentration: 3.75ug/ml.
Immunofluorescence: Strong expression of the protein seen in the nuclei of HeLa and U2OS cells. Recommended concentration: 10ug/ml.
Flow Cytometry: Flow cytometric analysis of HEK293 cells. Recommended concentration: 10ug/ml.
- O'Leary DA, Koleski D, Kola I, Hertzog PJ, Ristevski S. Identification and expression analysis of alternative transcripts of the mouse GA-binding protein (Gabp) subunits alpha and beta1. Gene. 2005 Jan 3;344:79-92. Epub 2004 Nov 23.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
500 ug/mL
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Expected Species Reactivity based on sequence homology: Dog
The immunogen for this antibody is: C-SSENSSKATDETG
2553
GA binding protein transcription factor, beta subunit 1
GABPB2
Homo sapiens
Liquid
NP_005245.2, NP_002032.2, NP_001307844.1
8051593
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Cancer
.
Q06547