Further Information
SGCG, sarcoglycan, gamma (35kDa dystrophin-associated glycoprotein), A4, DAGA4, DMDA, DMDA1, LGMD2C, MAM, MGC130048, SCARMD2, SCG3, TYPE, 35kD dystrophin-associated glycoprotein, Maghrebian myopathy (autosomal recessive), OTTHUMP00000018112, gamma sarcoglycan, limb girdle muscular dystrophy 2C (Duchenne-like muscular dystrophy, autosomal recessive), sarcoglycan, gamma (35kD dystrophin-associated glycoprotein)
Peptide ELISA: antibody detection limit dilution 1:2000.Western Blot:Preliminary experiments gave an approx 45kDa band in Human Heart lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 32.4kDa according to NP_000222.1. The 45kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
- Rafii MS, Hagiwara H, Mercado ML, Seo NS, Xu T, Dugan T, Owens RT, Hook M, McQuillan DJ, Young MF, Fallon JR, Biglycan binds to alpha- and gamma-sarcoglycan and regulates their expression during development. Journal of cellular physiology 2006 Nov 209 (2): 439-47.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
500 ug/mL
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Expected Species Reactivity based on sequence homology: Human
The immunogen for this antibody is: C-NQQFQINSNDGKP
6445
sarcoglycan, gamma (35kDa dystrophin-associated glycoprotein)
SGCG
Homo sapiens
Liquid
NP_000222.1
4557847
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Signal Transduction
Q13326