Further Information
GATA1, GATA binding protein 1 (globin transcription factor 1), HGNC:4170, ERYF1, GF1, NFE1, GATA binding protein 1, GATA-binding protein 1 (globin transcription factor 1), erythrold transcription factor 1, globin transcription factor 1, transcription factor GATA1, GF-1, XLTT, NF-E1 DNA-binding protein, erythroid transcription factor 1
Peptide ELISA: antibody detection limit dilution 1:16000.
Western Blot:Approx 50-55kDa band observed in nuclear lysates of cell line K562 calculated MW of 42.8kDa according to NP_002040.1). The observed molecular weight corresponds to different antibodies from other commercial sources. Recommended concentration: 0.3-1ug/ml. Primary incubation 1 hour at room temperature.
Negative Control: Human Hippocampus lysate.
Immunofluorescence: Strong expression of the protein seen in the nuclei of HeLa and NIH3T3 cells. Recommended concentration: 10ug/ml.
Flow Cytometry: Flow cytometric analysis of K562 cells. Recommended concentration: 10ug/ml.
- Li Z, Godinho FJ, Klusmann JH, Garriga-Canut M, Yu C, Orkin SH. Developmental stage-selective effect of somatically mutated leukemogenic transcription factor GATA1. Nat Genet. 2005 Jun;37(6):613-9. Epub 2005 May 15.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
500 ug/mL
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Expected Species Reactivity based on sequence homology: Rat, Dog, Cow
The immunogen for this antibody is: C-DAEAYRHSPVFQ
2623
GATA binding protein 1 (globin transcription factor 1)
GATA1
Homo sapiens
Liquid
NP_002040.1
4503925
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Cancer
.
P15976