ProSci

GAPDH Monoclonal Antibody

Product Code:
 
PSI-32-159
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Mouse
Antibody Isotype:
 
IgG1
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
2D4A7
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
 
GAPDH monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis of GAPDH using Hela, K562, Jurkat, NIH / 3T3, HepG2 cell lysate, and human stomach carcinoma tissue lysate .
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Western blot analysis of GAPDH using Hela, K562, Jurkat, NIH / 3T3, HepG2 cell lysate, and human stomach carcinoma tissue lysate .

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PSI-32-159-0.1mL0.1mL£452.00
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Further Information

Additional Names:
GAPD, CDABP0047, OK/SW-cl.12, Peptidyl-cysteine S-nitrosylase GAPDH, GAPDH, G3PD, GAPD, HEL-S-162eP
Application Note:
Western Blot:1:500 - 1:2,000
IHC(P/F):1:200 - 1:1,000
IF:1:200 - 1:1,000
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.
Background:
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. It catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Besides its functioning as a glycolytic enzyme in cytoplasm, recent evidence suggest that mammalian GAPDH is also involved in a great number of intracellular proceses such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, DNA replication, and DNA repair. During the last decade a lot of findings appeared concerning the role of GAPDH in different pathologies including prostate cancer progression, programmed neuronal cell death, age- related neuronal diseases, such as Alzheimer?s and Huntington?s disease.
Background References:
  • Allen R.W. J. Biol. Chem. 1987.262:649-653.
  • Sumner CJ. Ann Neurol 2003.54:6 47-54.
Buffer:
Ascitic fluid containing 0.03% sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Ni-NTA purified recombinant human GAPDH expressed in E. Coli strain BL21 (DE3).
NCBI Gene ID #:
2597
NCBI Official Name:
glyceraldehyde-3-phosphate dehydrogenase
NCBI Official Symbol:
GAPDH
NCBI Organism:
Homo sapiens
Protein Accession #:
P04406
Protein GI Number:
120649
Research Area:
Neuroscience
Swissprot #:
P04406
User NOte:
Optimal dilutions for each application to be determined by the researcher.