GAPDH Monoclonal Antibody
Antibody Clonality:
Monoclonal
Applications:- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Storage:
GAPDH monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
No additional charges, what you see is what you pay! *
Stay in control of your spending. These prices have no additional charges, not even shipping!
* Rare exceptions are clearly labelled (only 0.14% of items!).
Multibuy discounts available!
Contact us to find what you can save.
This product comes from:
United States.
Typical lead time:
14-21 working days.
Contact us for more accurate information.
- Further Information
- Documents
- Show All
Further Information
GAPD, CDABP0047, OK/SW-cl.12, Peptidyl-cysteine S-nitrosylase GAPDH, GAPDH, G3PD, GAPD, HEL-S-162eP
Western Blot:1:500 - 1:2,000
IHC(P/F):1:200 - 1:1,000
IF:1:200 - 1:1,000
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. It catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Besides its functioning as a glycolytic enzyme in cytoplasm, recent evidence suggest that mammalian GAPDH is also involved in a great number of intracellular proceses such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, DNA replication, and DNA repair. During the last decade a lot of findings appeared concerning the role of GAPDH in different pathologies including prostate cancer progression, programmed neuronal cell death, age- related neuronal diseases, such as Alzheimer?s and Huntington?s disease.
- Allen R.W. J. Biol. Chem. 1987.262:649-653.
- Sumner CJ. Ann Neurol 2003.54:6 47-54.
Ascitic fluid containing 0.03% sodium azide.
batch dependent
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Ni-NTA purified recombinant human GAPDH expressed in E. Coli strain BL21 (DE3).
2597
glyceraldehyde-3-phosphate dehydrogenase
GAPDH
Homo sapiens
P04406
120649
Neuroscience
P04406
Optimal dilutions for each application to be determined by the researcher.