Further Information
SCP2 , sterol carrier protein 2, DKFZp686C12188, DKFZp686D11188, NLTP, NSL-TP, SCPX, nonspecific lipid-transfer protein, sterol carrier protein X
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments gave an approx 50kDa band in Human Liver lysates after 0.3ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 59.0kDa according to NP_001007099.1 and of 6.7kDa according to NP_002970.2. The 28kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?Immunohistochemistry:In paraffin embedded Human Liver shows speckled cytoplasm staining in hepatocytes. Recommended concentration, 3-6ug/ml.
- Atshaves BP, Gallegos AM, McIntosh AL, Kier AB, Schroeder F. Sterol carrier protein-2 selectively alters lipid composition and cholesterol dynamics of caveolae/lipid raft vs nonraft domains in L-cell fibroblast plasma membranes. Biochemistry. 2003 Dec 16;42(49):14583-98.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
500 ug/mL
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Pig, Cow
The immunogen for this antibody is: C-KNHKHSVNNPYSQ
6342
sterol carrier protein 2
SCP2
Homo sapiens
Liquid
NP_001007099.1, NP_002970.2, NP_001180528.1, NP_001180529.1, NP_001180546.1
55956775
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Homeostasis
This antibody is expected to recognize isoform 1, 2, 6, 7 and 8 (NP_002970.2; NP_001007099.1; NP_001180529.1; NP_001180528.1; NP_001180546.1 respectively),
P22307