ProSci

Sterol Carrier Protein 2 Antibody

Product Code:
 
PSI-46-903
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Caprine (Goat)
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Enzyme-Linked Immunosorbent Assay (ELISA)
Storage:
 
Aliquot and store at -20˚C. Minimize freezing and thawing.
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46-903 (3.8ug/ml) staining of paraffin embedded Human Liver-. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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46-903 (3.8ug/ml) staining of paraffin embedded Human Liver-. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

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PSI-46-903-0.1mg0.1mg£626.00
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Further Information

Additional Names:
SCP2 , sterol carrier protein 2, DKFZp686C12188, DKFZp686D11188, NLTP, NSL-TP, SCPX, nonspecific lipid-transfer protein, sterol carrier protein X
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments gave an approx 50kDa band in Human Liver lysates after 0.3ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 59.0kDa according to NP_001007099.1 and of 6.7kDa according to NP_002970.2. The 28kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?Immunohistochemistry:In paraffin embedded Human Liver shows speckled cytoplasm staining in hepatocytes. Recommended concentration, 3-6ug/ml.
Background References:
  • Atshaves BP, Gallegos AM, McIntosh AL, Kier AB, Schroeder F. Sterol carrier protein-2 selectively alters lipid composition and cholesterol dynamics of caveolae/lipid raft vs nonraft domains in L-cell fibroblast plasma membranes. Biochemistry. 2003 Dec 16;42(49):14583-98.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: C-KNHKHSVNNPYSQ
NCBI Gene ID #:
6342
NCBI Official Name:
sterol carrier protein 2
NCBI Official Symbol:
SCP2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_001007099.1, NP_002970.2, NP_001180528.1, NP_001180529.1, NP_001180546.1
Protein GI Number:
55956775
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Homeostasis
SPECIFICITY:
This antibody is expected to recognize isoform 1, 2, 6, 7 and 8 (NP_002970.2; NP_001007099.1; NP_001180529.1; NP_001180528.1; NP_001180546.1 respectively),
Swissprot #:
P22307