Antibody Clonality:
Polyclonal
Applications:- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Storage:
Store antibody at -20˚C for up to one year.
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![Western blot analysis of lysed extracts from 293 cells untreated (Lane 1) or treated with serum (lane 2) using GSK3α (Phospho-Ser21). Western blot analysis of lysed extracts from 293 cells untreated (Lane 1) or treated with serum (lane 2) using GSK3α (Phospho-Ser21).](https://www.prosci-inc.com/static-images/79-105.jpg)
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![<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using GSK3α (Phospho-Ser21).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic staining using GSK3α (Phospho-Ser21). <b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using GSK3α (Phospho-Ser21).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic staining using GSK3α (Phospho-Ser21).](https://www.prosci-inc.com/static-images/79-105a.jpg)
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This product comes from:
United States.
Typical lead time:
14-21 working days.
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Further Information
MAPTL, MTBT1, TAU, Factor A, GSK-3 alpha, kinase GSK3-alpha
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN.
- Barry FA, et al. (2003) FEBS Lett. 553 (1-2): 173-178. Koivisto L, et al. (2003) J Cell Sci. 116 (Pt 18): 3749-3760. Welsh G I, et al. (1996) Trends Cell Biol. 6:274-279. Srivastava A K, et al. (1998) Mol. Cell. Biochem. 182: 135-141.
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
1 mg/mL
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
GSK3a (Phospho-Ser21) antibody was raised against a peptide sequence around phosphorylation site of serine 21 (T-S-S (p) -F-A) derived from Human GSK3α.
Phospho-Specific
2931
glycogen synthase kinase 3 alpha
GSK3A
Homo sapiens
Liquid
PREDICTED MOLECULAR WEIGHT:
51 kDa
NP_063937.2
49574532
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Phospho-Specific,Apoptosis,Cancer,Cell Cycle
This antibody detects endogenous level of GSK3α only when phosphorylated at serine 21and does not detect GSK-3β when phosphorylated at Ser9.
P49840