ProSci

NDUFA10 Antibody

Product Code:
 
PSI-XW-7881
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Chicken
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
 
NDUFA10 antibody can be stored at 4˚C for short term (weeks). Long term storage should be at -20˚C. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures
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Direct Elisa Test. Protein as test antigen. Affi-pure IgY as primary antibody and Goat anti-IgY HRP as 2nd antibody. Fixed amount of antigen (5 ug/mL) and serial dilutions of antibody. <br><br>E coli-derived fusion protein as test antigen. Affinity-purified IgY dilution: 1:2000, Goat anti-IgY-HRP dilution: 1:1000. Colorimetric method for signal development.
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Direct Elisa Test. Protein as test antigen. Affi-pure IgY as primary antibody and Goat anti-IgY HRP as 2nd antibody. Fixed amount of antigen (5 ug/mL) and serial dilutions of antibody. <br><br>E coli-derived fusion protein as test antigen. Affinity-purified IgY dilution: 1:2000, Goat anti-IgY-HRP dilution: 1:1000. Colorimetric method for signal development.

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CodeSizePrice
PSI-XW-7881-0.05mg0.05mg£801.00
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This product comes from: United States.
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Further Information

Additional Names:
Complex I-42kD, CI-42kD, NADHubiquinone oxidoreductase 42 kDa subunit, mitochondrial, Complex I-42KD, CI-42KD
Application Note:
NADH-ubiquinone oxidoreductase 42 kDa subunit, mitochondrial antibody can be used for the detection of NADH-ubiquinone oxidoreductase 42 kDa subunit, mitochondrial by ELISA and Western Blot, may also work for ICC and IHC.
Background:
FUNCTION: Transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

CATALYTIC ACTIVITY: NADH + acceptor = NAD+ + reduced acceptor.

SUBUNIT: Mammalian complex I is composed of 45 different subunits. This a component of the hydrophobic protein fraction.

COFACTOR: Binds 1 FAD per subunit.

SUBCELLULAR LOCATION: Mitochondrion; mitochondrial matrix.
Buffer:
Phosphate-Buffered Saline. No preservatives added.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
21-140
NCBI Gene ID #:
4705
NCBI Official Name:
NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 10, 42kDa
NCBI Official Symbol:
NDUFA10
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
40.8 kDa (calculated)
Protein Accession #:
NP_004535.1
Protein GI Number:
4758768
Purification:
Immunoaffinity Purified
Swissprot #:
O95299
User NOte:
Optimal dilutions for each application to be determined by the researcher.