ProSci

ZNF265 Antibody

Product Code:
 
PSI-28-738
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Canine (dog)
  • Fish
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
 
For short periods of storage (days) store at 4˚C. For longer periods of storage, store ZNF265 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
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Antibody used in WB on Human HepG2 at 1.25 ug/ml.
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Antibody used in WB on Human HepG2 at 1.25 ug/ml.

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PSI-28-738-100ul100ul£609.00
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Further Information

Additional Names:
ZNF265, ZIS, ZIS1, ZIS2, ZNF265
Application Note:
ZNF265 antibody can be used for detection of ZNF265 by ELISA at 1:312500. ZNF265 antibody can be used for detection of ZNF265 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Background:
ZNF265 is a protein that has been shown to bind to the spliceosomal components U1-70K and U2AF35 and to direct alternative splicing. Analysis of the structure reveals substantial similarity to known RNA-binding motifs in terms of the distribution of key surface residues responsible for making RNA contacts, despite a complete lack of structural homology. An RNA gel shift assay was used to demonstrate that a single crossed finger domain from ZNF265 is capable of binding to an RNA message. Taken together, these results define a new RNA-binding motif and should provide insight into the functions of the >100 uncharacterized proteins in the sequence data bases that contain this domain.
Background References:
  • Plambeck, C.A., et al., (2003) J. Biol. Chem. 278 (25), 22805-22811.
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ZNF265.
NCBI Gene ID #:
9406
NCBI Official Name:
zinc finger, RAN-binding domain containing 2
NCBI Official Symbol:
ZRANB2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
37 kDa, 36 kDa
Protein Accession #:
NP_976225
Protein GI Number:
42741684
Purification:
Antibody is purified by peptide affinity chromatography method.
Research Area:
Transcription
Swissprot #:
Q5VV34
User NOte:
Optimal dilutions for each application to be determined by the researcher.