ProSci

CRISP2 Antibody

Product Code:
 
PSI-42-520
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Caprine (Goat)
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
 
Aliquot and store at -20˚C. Minimize freezing and thawing.
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HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human CRISP2 (RC205312) with C-terminal MYC tag probed with 42-520 (1ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with 42-520 (1mg/ml) in Lane
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HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human CRISP2 (RC205312) with C-terminal MYC tag probed with 42-520 (1ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with 42-520 (1mg/ml) in Lane

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PSI-42-520-0.1mg0.1mg£626.00
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Further Information

Additional Names:
CRISP2, cysteine-rich secretory protein 2, CRISP-2, GAPDL5, MGC111136, TPX1, TSP1, OTTHUMP00000016589, glyceraldehyde-3-phosphate dehydrogenase-like 5, testis specific protein 1 (probe H4-1 p3-1)
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:In transfected HEK293 transiently expressing Human CRISP2 (MYC and DYKDDDDK tagged), a band of approx. 35kDa was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-myc tag antibody. Recommended concentration: 0.5-1ug/ml. Primary incubations were for 1 hour. Preliminary experiments gave an approx 55kDa band in Human Testis lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 27.3kDa according to NP_003287.1. The 55kDa band was successfully blocked by incubation with the immunizing peptide.Immunohistochemistry:Paraffin embedded Human Testis. Recommended concentration: 3.75ug/ml.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Pig, Dog
Immunogen:
The immunogen for this antibody is: C-QHSDPEDRKTSTR
NCBI Gene ID #:
7180
NCBI Official Name:
cysteine-rich secretory protein 2
NCBI Official Symbol:
CRISP2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_003287.1
Protein GI Number:
4507671
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
SPECIFICITY:
Reported variants represent identical protein: NP_003287.1, NP_001135879.1, NP_001135889.1, NP_001135907.1, NP_001135880.1.
Swissprot #:
P16562