ProSci

DGCR8 Antibody

Product Code:
 
PSI-42-075
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Caprine (Goat)
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Enzyme-Linked Immunosorbent Assay (ELISA)
Storage:
 
Aliquot and store at -20˚C. Minimize freezing and thawing.
 

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PSI-42-075-0.1mg0.1mg£505.00
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Further Information

Additional Names:
DGCR8, DiGeorge syndrome critical region gene 8, C22orf12, DGCRK6, Gy1, pasha
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments gave an approx. 75kDa band in Human Brain (amygdala) lysates after 0.2ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 86.0kDa according to NP_073557.3. The 75kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Background References:
  • Stark KL, Xu B, Bagchi A, Lai WS, Liu H, Hsu R, Wan X, Pavlidis P, Mills AA, Karayiorgou M, Gogos JA. Altered brain microRNA biogenesis contributes to phenotypic deficits in a 22q11-deletion mouse model. Nat. Genet. 2008 Jun 40 (6): 751-60.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Human, Mouse, Rat, Cow
Immunogen:
The immunogen for this antibody is: C-KRFDFEQVTVKKFRT
NCBI Gene ID #:
54487
NCBI Official Name:
DGCR8 microprocessor complex subunit
NCBI Official Symbol:
DGCR8
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_073557.3
Protein GI Number:
38488720
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Homeostasis
Swissprot #:
Q8WYQ5