ProSci

Exosome Component 9 Antibody

Product Code:
 
PSI-46-967
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Caprine (Goat)
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
 
Aliquot and store at -20˚C. Minimize freezing and thawing.
1 / 1
46-967 (0.3ug/ml) staining of Jurkat lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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46-967 (0.3ug/ml) staining of Jurkat lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

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PSI-46-967-0.1mg0.1mg£626.00
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This product comes from: United States.
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Further Information

Additional Names:
EXOSC9, exosome component 9, PM/Scl-75, PMSCL1, RRP45, Rrp45p, p5, p6, P75 polymyositis-scleroderma overlap syndrome associated autoantigen, PMSCL autoantigen, 75kD, autoantigen PM/Scl 1, polymyositis/scleroderma autoantigen 1 (75kD), polymyositis/scleroderma autoantigen 1, 75kDa
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx. 75kDa band observed in lysates of cell line Jurkat (calculated MW of 50.8kDa according to NP_001029366.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Alderuccio, J Exp Med. 1991 Apr 1;173(4):941-52; PMID: 2007859). Recommended concentration: 0.3-1ug/ml.
Background References:
  • Schilders G, Raijmakers R, Malmegrim KC, Vande Walle L, Saelens X, Vree Egberts W, van Venrooij WJ, Vandenabeele P, Pruijn GJ. Caspase-mediated cleavage of the exosome subunit PM/Scl-75 during apoptosis. Arthritis Res Ther. 2007;9(1):R12.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-RTQTTSAKQEKAP
NCBI Gene ID #:
5393
NCBI Official Name:
exosome component 9
NCBI Official Symbol:
EXOSC9
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_001029366.1, NP_005024.2
Protein GI Number:
77812672
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
SPECIFICITY:
This antibody is expected to recognize both reported isoforms (NP_001029366.1; NP_005024.2).
Swissprot #:
Q06265