Further Information
GLUD1, glutamate dehydrogenase 1, GDH, GDH1, GLUD, MGC132003,
glutamate dehydrogenase (NAD(P)+), GLUD2, glutamate dehydrogenase 2, GDH2, GLUDP1, glutamate dehydrogenase pseudogene 1
Peptide ELISA: antibody detection limit dilution 1:32000.
Western Blot:Approx 55kDa band observed in Human Brain (Cerebellum) and Mouse Brain lysates and in Human, Mouse and Rat Liver lysates (calculated MW of 61.4kDa according to Human NP_005262.1, of 61.3kDa according to Mouse NP_032159.1 and of 61.4kDa according to Rat NP_036702.1). Recommended concentration: 0.3-1ug/ml. Primary incubation was 1 hour.
Immunohistochemistry:In paraffin embedded Human Cerebral Cortex shows mitochondrial staining in neuronal cell bodies and axons. Recommended concentration: 3-5ug/ml.
Immunofluorescence: Customer found particulate cytoplasm staining in HeLa.
Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
500 ug/mL
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Expected Species Reactivity based on sequence homology: Dog, Cow
The immunogen for this antibody is: C-ESEEQKRNRVRGILR
2746; 2747
glutamate dehydrogenase 1
GLUD1 / GLUD2
Homo sapiens
Liquid
NP_005262.1, NP_036216.2
4885281
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Neuroscience ,Signal Transduction
This antibody is expected to recognize both reported isoforms (NP_005262.1; NP_036216.2).
P00367