ProSci

GLUD1 / GLUD2 Antibody

Product Code:
 
PSI-42-524
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Caprine (Goat)
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
 
Aliquot and store at -20˚C. Minimize freezing and thawing.
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42-524 (0.03ug/ml) staining of Human (A) and Mouse (B) Brain lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
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42-524 (0.03ug/ml) staining of Human (A) and Mouse (B) Brain lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

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PSI-42-524-0.1mg0.1mg£626.00
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This product comes from: United States.
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Further Information

Additional Names:
GLUD1, glutamate dehydrogenase 1, GDH, GDH1, GLUD, MGC132003, glutamate dehydrogenase (NAD(P)+), GLUD2, glutamate dehydrogenase 2, GDH2, GLUDP1, glutamate dehydrogenase pseudogene 1
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 55kDa band observed in Human Brain (Cerebellum) and Mouse Brain lysates and in Human, Mouse and Rat Liver lysates (calculated MW of 61.4kDa according to Human NP_005262.1, of 61.3kDa according to Mouse NP_032159.1 and of 61.4kDa according to Rat NP_036702.1). Recommended concentration: 0.3-1ug/ml. Primary incubation was 1 hour.Immunohistochemistry:In paraffin embedded Human Cerebral Cortex shows mitochondrial staining in neuronal cell bodies and axons. Recommended concentration: 3-5ug/ml.Immunofluorescence: Customer found particulate cytoplasm staining in HeLa.

Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Dog, Cow
Immunogen:
The immunogen for this antibody is: C-ESEEQKRNRVRGILR
NCBI Gene ID #:
2746; 2747
NCBI Official Name:
glutamate dehydrogenase 1
NCBI Official Symbol:
GLUD1 / GLUD2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_005262.1, NP_036216.2
Protein GI Number:
4885281
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Neuroscience ,Signal Transduction
SPECIFICITY:
This antibody is expected to recognize both reported isoforms (NP_005262.1; NP_036216.2).
Swissprot #:
P00367