ProSci

ORC4L Antibody

Product Code:
 
PSI-46-102
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Caprine (Goat)
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
Applications:
  • ChIP
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
 
Aliquot and store at -20˚C. Minimize freezing and thawing.
1 / 1
46-102 (0.3ug/ml) staining of Jurkat nuclear lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
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46-102 (0.3ug/ml) staining of Jurkat nuclear lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

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PSI-46-102-0.1mg0.1mg£626.00
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Further Information

Additional Names:
ORC4L, origin recognition complex, subunit 4-like (yeast), Orc4p, HSORC4, HsORC4, origin recognition complex, subunit 4 (yeast homolog)-like, Origin recognition complex, subunit 4, S. cerevisiae, homolog-like, ORC4, ORC4P, origin recognition complex subunit 4
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 48kDa band observed in nuclear lysates of cell line Jurkat (calculated MW of 50.4kDa according to Human NP_002543.2 Recommended concentration: 0.3-1ug/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on cell line NIH-3T3 and Mouse Heart for this particular batch.
Background References:
  • Vashee S, Simancek P, Challberg MD, Kelly TJ. Assembly of the human origin recognition complex. J Biol Chem 2001 Jul 13;276(28):26666-73
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: C-DVRQWATSSLSWL
NCBI Gene ID #:
5000
NCBI Official Name:
origin recognition complex, subunit 4
NCBI Official Symbol:
ORC4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_002543.2, NP_001177810.1, NP_001177811.1
Protein GI Number:
32454750
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
This antibody is expected to recognize all reported isoforms (NP_002543.2; NP_001177810.1; NP_001177811.1). Reported variants represent identical protein: NP_002543.2, NP_001177808.1, NP_859526.1, NP_859525.1
Swissprot #:
O43929