ProSci

PCBP4 Antibody

Product Code:
 
PSI-46-143
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Caprine (Goat)
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
 
Aliquot and store at -20˚C. Minimize freezing and thawing.
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46-143 (0.03ug/ml) staining of HeLa cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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46-143 (0.03ug/ml) staining of HeLa cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

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PSI-46-143-0.1mg0.1mg£626.00
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Further Information

Additional Names:
PCBP4, poly(rC) binding protein 4 , LIP4, MCG10 , LYST-interacting protein, RNA binding protein MCG10, alphaCP-4 protein, poly(rC)-binding protein 4, alpha CP-4,
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx. 40kDa band observed in lysates of cell line HeLa (calculated MW of 41.4kDa according to NP_127502.1 and NP_127503.1). In transfected HEK293 transiently expressing PCBP4 a band of approx. 45kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 1-3ug/ml.
Background References:
  • Pio R, Zudaire I, Pino I, Castano Z, Zabalegui N, Vicent S, Garcia-Amigot F, Odero MD, Lozano MD, Garcia-Foncillas J, Calasanz MJ, Montuenga LM. Alpha CP-4, encoded by a putative tumor suppressor gene at 3p21, but not its alternative splice variant alpha CP-4a, is underexpressed in lung cancer. Cancer Res. 2004 Jun 15;64(12):4171-9.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog
Immunogen:
The immunogen for this antibody is: C-NGSKKAERQKFSPY
NCBI Gene ID #:
57060
NCBI Official Name:
poly(rC) binding protein 4
NCBI Official Symbol:
PCBP4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_065151.1, NP_127501.1, NP_127502.1, NP_127503.1
Protein GI Number:
9966803
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
This antibody is expected to recognise all reported isoforms (NP_065151.1, NP_127501.1, NP_127502.1 and NP_127503.1).
Swissprot #:
P57723