Host Type:
Caprine (Goat)
Antibody Clonality:
Polyclonal
Applications:- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.
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This product comes from:
United States.
Typical lead time:
14-21 working days.
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- Further Information
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Further Information
PCK1, phosphoenolpyruvate carboxykinase 1 (soluble), HGNC:8724, MGC22652, PEPCK1, PEPCKC, PEP carboxykinase, cytosolic phosphoenolpyruvate carboxykinase 1, phosphoenolpyruvate carboxylase, phosphopyruvate carboxylase, PEPCK-C
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Preliminary experiments gave bands at approx 70kDa, 45kDa and 28kDa in Human Adipose, Kidney and Liver lysates after 0.3 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the additional bands we observe given the calculated size of 69.2 kDa according to NP_002582.2. All three detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).Immunohistochemistry:Paraffin embedded Human Cerebral Cortex, Kidney and Liver. Recommended concentration: 2ug/ml.
- Yang Q, Graham TE, Mody N, Preitner F, Peroni OD, Zabolotny JM, Kotani K, Quadro L, Kahn BB. Serum retinol binding protein 4 contributes to insulin resistance in obesity and type 2 diabetes. Nature. 2005 Jul 21;436(7049):356-62.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
500 ug/mL
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Pig, Cow
The immunogen for this antibody is: C-HVNWFRKDKEGK
5105
phosphoenolpyruvate carboxykinase 1 (soluble)
PCK1
Homo sapiens
Liquid
NP_002582.3
32483401
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Cancer
.
P35558