Further Information
PFKFB2, 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2, DKFZp781D2217, MGC138308, MGC138310, PFK-2/FBPase-2, 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2, 6PF-2-K/Fru-2,6-P2ASE heart-type isozyme, OTTHUMP00000034285, OTTHUMP00000034286, PFKFB, cardiac, fructose-2,6-bisphosphatase, cardiac isozyme
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Preliminary experiments gave an approx 75Da band in Human Heart lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 58.5kDa according to NP_006203.2 and 54.4kDa according to NP_001018063.1. The 75kDa band was successfully blocked by incubation with the immunizing peptide.Immunohistochemistry:Paraffin embedded Human Spleen. Recommended concentration: 2.5ug/ml.
- Arden C, Hampson LJ, Huang GC, Shaw JA, Aldibbiat A, Holliman G, Manas D, Khan S, Lange AJ, Agius L, A role for PFK-2/FBPase-2, as distinct from fructose 2, 6-bisphosphate, in regulation of insulin secretion in pancreatic beta-cells. Biochem J. 2008 Apr 1;411(1):41-51.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
500 ug/mL
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Expected Species Reactivity based on sequence homology: Dog, Cow
The immunogen for this antibody is: SEQNNNSYETKTP-C
5208
6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2
PFKFB2
Homo sapiens
Liquid
NP_006203.2, NP_001018063.1
64762406
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Signal Transduction ,Cancer
This antibody is expected to recognize both reported isoforms (NP_006203.2; NP_001018063.1).
O60825