Further Information
PLA2G4A, phospholipase A2, group IVA (cytosolic, calcium-dependent), MGC126350, PLA2G4, cPLA2-alpha, calcium-dependent phospholipid-binding protein, cytosolic phospholipase A2, group IVA, lysophospholipase, phosphatidylcholine 2-acylhydrolase
Peptide ELISA: antibody detection limit dilution 1:32000.
Western Blot:Approx 80-85kDa band observed in lysates of cell line A549 (calculated MW of 85.2kDa according to NP_077734.1). Preliminary experiments also showed an 85- 90kDa band in lysates of cell lines A431, HeLa and NIH-3T3 Recommended concentration:0.3-1ug/ml. Primary incubation 1 hour at room temperature.Preliminary testing was unsuccessful on Rat for this particular batch.
Immunohistochemistry:Paraffin embedded Human Uterus. Recommended concentration: 3.75ug/ml.
Immunofluorescence: Strong expression of the protein seen in the cytoplasm of A549 cells. Recommended concentration: 10ug/ml.
Flow Cytometry: Flow cytometric analysis of A549 cells. Recommended concentration: 10ug/ml.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
500 ug/mL
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Expected Species Reactivity based on sequence homology: Mouse, Rat
The immunogen for this antibody is: C-QEKTFRQQRKEHIR
5321
phospholipase A2, group IVA (cytosolic, calcium-dependent)
PLA2G4A
Homo sapiens
Liquid
NP_077734.1
23943920
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Signal Transduction ,Immunology
.
P47712