ProSci

POU5F1 Antibody

Product Code:
 
PSI-42-580
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Caprine (Goat)
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
 
Aliquot and store at -20˚C. Minimize freezing and thawing.
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42-580 (1ug/ml) staining of NIH3T3 cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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42-580 (1ug/ml) staining of NIH3T3 cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

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PSI-42-580-0.1mg0.1mg£626.00
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This product comes from: United States.
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Further Information

Additional Names:
Pou5f1, POU domain, class 5, transcription factor 1, Oct-3, Oct-3/4, Oct-4, Oct3, Oct3/4, Oct4, Otf-3, Otf-4, Otf3, Otf3-rs7, Otf3g, Otf4
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 38-40kDa band observed in lysates of cell line NIH3T3 and approx. 45kDa in lysates of cell line HepG2 (calculated MW of 38.6kDa according to Human NP_002692.2 and 38.2kDa according to Mouse NP_038661.2). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 1-2ug/ml.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Rat
Immunogen:
The immunogen for this antibody is: C-DRPNAVKLEKVEP
NCBI Gene ID #:
18999 (mouse);
NCBI Official Name:
POU domain, class 5, transcription factor 1
NCBI Official Symbol:
POU5F1
NCBI Organism:
Mus musculus
Physical State:
Liquid
Protein Accession #:
NP_038661.2,
Protein GI Number:
125490392
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Transcription
SPECIFICITY:
.
Swissprot #:
P20263