Further Information
PTCH, patched homolog (Drosophila), RP11-435O5.3, BCNS, FLJ42602, HPE7, NBCCS, PTC, PTC1, PTCH1 , PTCH protein +12b, PTCH protein +4', PTCH protein -10, patched, patched (Drosophila) homolog
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 150kDa band observed in Human Brain lysates (calculated MW of 153.9kDa according to NP_001077071.1). Recommended concentration: 1-3ug/ml. Primary incubation 1 hour at room temperature. Immunofluorescence :Expression of the protein seen in the Golgi/membrane of NIH3T3 cells. Recommended concentration: 5ug/ml.
- Nagao K, Togawa N, Fujii K, Uchikawa H, Kohno Y, Yamada M, Miyashita T. Detecting tissue-specific alternative splicing and disease-associated aberrant splicing of the PTCH gene with exon junction microarrays. Hum Mol Genet. 2005 Nov 15;14(22):3379-88. Epub 2005 Oct 3.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
500 ug/mL
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Expected Species Reactivity based on sequence homology: Dog, Pig, Cow
The immunogen for this antibody is: C-HPESRHHPPSNPRQQ
5727
patched 1
PTCH1
Homo sapiens
Liquid
NP_000255.2, NP_001077072.1, NP_001077071.1, NP_001077075.1
4506247
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Cancer
This antibody is expected to recognise all four reported isoforms (NP_000255.2; NP_001077072.1; NP_001077071.1; NP_001077075.1). Reported variants represent identical protein (NP_001077075.1; NP_001077076.1; NP_001077073.1; NP_001077074.1).
Q13635