ProSci

RBM3 Antibody

Product Code:
 
PSI-42-084
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Caprine (Goat)
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
 
Aliquot and store at -20˚C. Minimize freezing and thawing.
1 / 1
42-084 (0.01ug/ml) staining of HepG2 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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42-084 (0.01ug/ml) staining of HepG2 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

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PSI-42-084-0.1mg0.1mg£626.00
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Further Information

Additional Names:
RBM3, RNA binding motif (RNP1, RRM) protein 3, IS1-RNPL, RNPL, OTTHUMP00000025800, OTTHUMP00000025802, RNA binding motif protein 3
Application Note:
Peptide ELISA: antibody detection limit dilution 1:2000.Western Blot:Approx.17kDa band observed in lysates of cell line HepG2 (calculated MW of 17.2kDa according to NP_006734.1). Recommended concentration: 0.01-0.03ug/ml. An additional band of unknown identity was also consistently observed at 48kDa. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Background References:
  • Sureban SM, Ramalingam S, Natarajan G, May R, Subramaniam D, Bishnupuri KS, Morrison AR, Dieckgraefe BK, Brackett DJ, Postier RG, Houchen CW, Anant S. Translation regulatory factor RBM3 is a proto-oncogene that prevents mitotic catastrophe. Oncogene. 2008 Jul 31;27(33):4544-56.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog
Immunogen:
The immunogen for this antibody is: C-DRYSGGNYRDNYDN
NCBI Gene ID #:
5935
NCBI Official Name:
RNA binding motif (RNP1, RRM) protein 3
NCBI Official Symbol:
RBM3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_006734.1
Protein GI Number:
5803137
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
Swissprot #:
P98179