ProSci

RNase L Antibody

Product Code:
 
PSI-46-735
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Caprine (Goat)
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Enzyme-Linked Immunosorbent Assay (ELISA)
Storage:
 
Aliquot and store at -20˚C. Minimize freezing and thawing.
 

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PSI-46-735-0.1mg0.1mg£505.00
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Further Information

Additional Names:
ABCE1, ATP-binding cassette, sub-family E (OABP), member 1, ABC38, OABP, RLI, RNASEL1, RNASELI, RNS4I, ATP-binding cassette, sub-family E, member 1, RNase L inhibitor, ribonuclease L (2',5'-oligoisoadenylate synthetase-dependent) inhibitor
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments gave an approx. 80kDa band in Human Spleen lysates after 0.2ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 67.3kDa according to NP_002931.2. The ~80kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Background References:
  • Shichijo S, Ishihara Y, Azuma K, Komatsu N, Higashimoto N, Ito M, Nakamura T, Ueno T, Harada M, Itoh K. ABCE1, a member of ATP-binding cassette transporter gene, encodes peptides capable of inducing HLA-A2-restricted and tumor-reactive cytotoxic T lymphocytes in colon cancer patients. Oncol Rep. 2005 May;13(5):907-13.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Human, Mouse, Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: NHDKCKPKKCRQEC
NCBI Gene ID #:
6059
NCBI Official Name:
ATP-binding cassette, sub-family E (OABP), member 1
NCBI Official Symbol:
ABCE1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_002931.2, NP_001035809.1
Protein GI Number:
108773782
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Homeostasis
SPECIFICITY:
Reported variants represent identical protein: NP_002931.2 NP_001035809.1
Swissprot #:
P61221