ProSci

SAE1 Antibody

Product Code:
 
PSI-46-328
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Caprine (Goat)
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
 
Aliquot and store at -20˚C. Minimize freezing and thawing.
1 / 1
46-328 staining (1ug/ml) of Jurkat lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
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46-328 staining (1ug/ml) of Jurkat lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

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CodeSizePrice
PSI-46-328-0.1mg0.1mg£626.00
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This product comes from: United States.
Typical lead time: 14-21 working days.
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Further Information

Additional Names:
SAE1, AOS1, SUA1, HSPC140, SUMO-1 activating enzyme subunit 1, sentrin/SUMO-activating protein AOS1, SUMO-1 activating enzyme E1 N subunit, ubiquitin-like protein SUMO-1 activating enzyme, SUMO1 activating enzyme subunit 1, FLJ3091, UBLE1A, activator of SUMO1, ubiquitin-like 1-activating enzyme E1A
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 40kDa band observed in Jurkat and 293 cell lysates (predicted MW of 40kDa according to NP_005491). Recommended for use at 1ug/ml.
Background References:
  • Desterro JM, Rodriguez MS, Kemp GD, Hay RT. Identification of the enzyme required for activation of the small ubiquitin-like protein SUMO-1. J Biol Chem. 1999 Apr 9;274(15):10618-24.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Dog, Cow
Immunogen:
The immunogen for this antibody is: MKGNGIVECLGPK
NCBI Gene ID #:
10055
NCBI Official Name:
SUMO1 activating enzyme subunit 1
NCBI Official Symbol:
SAE1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_005491.1
Protein GI Number:
4885585
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
.
Swissprot #:
Q9UBE0