ProSci

SAE2 Antibody

Product Code:
 
PSI-46-329
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Caprine (Goat)
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Enzyme-Linked Immunosorbent Assay (ELISA)
Storage:
 
Aliquot and store at -20˚C. Minimize freezing and thawing.
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46-329 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. Actin filaments were stained wit
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46-329 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. Actin filaments were stained wit

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PSI-46-329-0.1mg0.1mg£626.00
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Further Information

Additional Names:
UBA2, SAE2, SUMO-1 activating enzyme subunit 2
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:This product has been successfully used in Western blot on Mouse: Kanemaru A, Saitoh H., Biosci Biotechnol Biochem. 2013;77(7):1575-8. PMID: 23832333.Immunofluorescence: Strong expression of the protein seen in the nuclei of U2OS and HeLa cells. Recommended concentration: 10ug/ml.
Background References:
  • Desterro JM, Rodriguez MS, Kemp GD, Hay RT. Identification of the enzyme required for activation of the small ubiquitin-like protein SUMO-1. J Biol Chem. 1999 Apr 9;274(15):10618-24.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Cow
Immunogen:
The immunogen for this antibody is: ALSRGLPRELAEA-C
NCBI Gene ID #:
10054
NCBI Official Name:
ubiquitin-like modifier activating enzyme 2
NCBI Official Symbol:
SAE2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_005490
Protein GI Number:
4885649
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
Swissprot #:
Q9UBT2