Further Information
SF3B3, SAP130, splicing factor 3b, subunit 3, 130kDa, RSE1, SF3B130, KIAA0017, STAF130, pre-mRNA splicing factor SF3b, 130 kDa subunit, spliceosome-associated protein 130, splicing factor 3b, subunit 3, SF3b130
Peptide ELISA: antibody detection limit dilution 1:2000.Western Blot:Approx 150kDa band observed in nuclear lysates of cell line HeLa (calculated MW of 136kDa according to NP_036558.3). Recommended concentration: 0.1-0.3ug/ml. Preliminary testing was unsuccessful on NIH3T3 for this particular batch. Primary incubated for 1 hour.
- Das BK, Xia L, Palandjian L, Gozani O, Chyung Y, Reed R. Characterization of a protein complex containing spliceosomal proteins SAPs 49, 130, 145, and 155. Mol Cell Biol 1999 Oct;19(10):6796-802
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
500 ug/mL
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Cow
The immunogen for this antibody is: C-VSKKLEDIRTRYAF
23450
splicing factor 3b, subunit 3, 130kDa
SF3B3
Homo sapiens
Liquid
NP_036558.3
54112121
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Cancer
.
Q15393