ProSci

LTF Antibody

Product Code:
 
PSI-46-745
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Caprine (Goat)
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
 
Aliquot and store at -20˚C. Minimize freezing and thawing.
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46-745 (0.05ug/ml) staining of Peripheral Blood Mononucleocyte lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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46-745 (0.05ug/ml) staining of Peripheral Blood Mononucleocyte lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

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PSI-46-745-0.1mg0.1mg£626.00
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This product comes from: United States.
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Further Information

Additional Names:
LTF, lactotransferrin, GIG12, HLF2, LF, growth-inhibiting protein 12, lactoferrin, neutrophil lactoferrin, talalactoferrin
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx. 85kDa band observed in Human Peripheral Mononucleocyte lysates (calculated MW of 78.2kDa according to NP_002334.2). Recommended concentration: 0.05-0.2ug/ml.Immunohistochemistry:In paraffin embedded blood vessel shows strong staining of cytoplasm of neutrophils.. Recommended concentration, 2-4ug/ml.Enzyme immunoassay: Sandwich-type ELISA with increasing amount of recombinant LTF captured by a rabbit antibody. Recommended reporter concentration: 5-10ug/ml
Background References:
  • de la Rosa G, Yang D, Tewary P, Varadhachary A, Oppenheim JJ. Lactoferrin acts as an alarmin to promote the recruitment and activation of APCs and antigen-specific immune responses. J Immunol. 2008 May 15;180(10):6868-76.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-ENYKSQQSSDPDP
NCBI Gene ID #:
4057
NCBI Official Name:
lactotransferrin
NCBI Official Symbol:
LTF
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_002334.2
Protein GI Number:
54607120
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Innate Immunity
SPECIFICITY:
.
Swissprot #:
P02788